2015-10-282014-08-22FREITAS, Ariane Cristine de. Estimativa da densidade dos perfis de corpos celulares de neurônios nos núcleos da base e avaliação comportamental de camundongos sob o uso de esteróides anabolizantes. 2014. 69 f. Dissertação (Mestrado em Biociências Aplicada à Saúde) - Universidade Federal de Alfenas, Alfenas, MG, 2014.https://repositorio.unifal-mg.edu.br/handle/123456789/689In the last decades, Anabolic-Androgenic Steroids (AAS) or Anabolic Steroids have been used mostly by elite athletes involved in sports that require strength and speed in order to improve their physical performance in competitions. Nevertheless, the abuse of these drugs has been also expanded to gym customers interested in body composition alterations seen by means of lean body mass increase and subcutaneous fat reduction. Few are known about AAS action in human brain, but there are evidences of changes in aggressive behavior, anxiety and depression. Thus, we aimed to study the neuronal loss by the use and abuse of AAS in mice. For doing so, we used 60 Swiss mice, 30 males and 30 females, proceeding from the Central Biotery of the Alfenas Federal University. A group of 20 animals was treated with testosterone cypionate (Deposteron®); another group of other 20 animals was treated with stanozolol (Winstrol Depot®); and a control group involving other 20 animals was treated with saline solution. All animals of the three groups were put under daily 15-minute swimming exercise during 30 days of treatment in an attempt to generate the same stress conditions that a physical exercise. After treatment, all animals were submitted to three behavioral tests: anxiety analysis, memory and recognition of objects and motor activity in the open field. Ending treatment and tests, the specimens were euthanized by halothane inhalation. Their brains were withdrawn and stored in 4% formaldehyde solution for three weeks. From each brain we took homotypic transverse section samples from its middle area to evaluate the areas proposed for our study. The results of estimated profile analyses of neuron cell bodies showed that there was a decrease of their number in pallidum of animals treated with Wintrol Depot®. Besides that, behavioral tests analyses confirmed that AAS administration in these animals caused anxiety and apathy and a decrease in the ability of recognizing objects; on the other hand, we do not observed change in motor ability nor in recent memory of animals so treated. Such results allowed to conclude that the abuse of AAS, without medical orientation, can lead us to cell degeneration, cerebral and cognitive activity loss and severe behavioral changes.application/pdfAcesso Abertohttp://creativecommons.org/licenses/by-nc-nd/4.0/AnabolizantesNeurôniosNucleos da basePesquisa comportamentalCIENCIAS BIOLOGICAS::MORFOLOGIADissertaçãoEsteves, Alessandra