2022-12-292023-02-062022-11-29SILVA, João Marcos Oliveira da.. Avaliação dos efeitos da inibição da histona desacetilase 6 em linhagens celulares de melanoma. 2022. 107 f. Dissertação (Mestrado em Biociências Aplicada à Saúde) - Universidade Federal de Alfenas, Alfenas, MG, 2022.https://repositorio.unifal-mg.edu.br/handle/123456789/2138Malignant melanoma, among the types of skin cancer, has the highest number of deaths and a propensity for rapid progression. Several studies have shown that HDAC6 overexpression contributes to the development of melanoma and a worse prognosis for the patient. For this reason, this work aims to investigate the impact of HDAC6 inhibition in melanoma cell lines. We used three melanoma cell lines (CHL-1, WM1366 and SK-MEL-147) to evaluate the effects of HDAC6 inhibition with the WT-161 inhibitor, using cell viability assays, clonogenic capacity of the cells, apoptotic cell death, cell cycle and cell motility analyzes. As a response to WT-161 treatment, we found a decrease in proliferative and clonogenic capacity, a decrease in chemoresistance, an increase in the apoptotic population, a decrease in migratory/invasive capacity with an increase in adhesion capacity. We believe that by inhibiting HDAC6 we were able to elucidate part of a complex network that promotes and supports tumor progression.application/pdfAcesso Embargadohttp://creativecommons.org/licenses/by-nc-nd/4.0/HDAC6WT-161MelanomaCâncerQuimiorresistênciaCIENCIAS DA SAUDEDissertaçãoGamero, Angel Mauricio Castro